A Comparative Study of Survival, Metabolism, Immune Indicators, and Proteomics, in Five Batches of Japanese Scallop Mizuhopecten yessoensis under Short-Term High Temperature Stress

Five batches of the Japanese scallop Mizuhopecten pyessoensis were tested for survival rate, oxygen consumption, catalase (CAT) and superoxide dismutase (SOD) activities, total antioxidant capacities (T-AOC) contents, and proteomics under short-term high temperature conditions. The five batches, (W1, W2, W3, W4, W5) selected from the established 21 ‘ivory white’ M. yessoensis batches, had higher survival rates than the other batches after one year of culture. Initial rearing water temperature of 15°C was increased by 1°C per day with a cooling and heating system. The temperature was raised until over 50% of the scallops from 3 batches died. This occurred at 30°C. The higher than normal culture temperature conditions showed significant or highly significant differences in the responses of some of the batches. Some showed significantly higher survival rates and significantly different rates of oxygen consumption. CAT activity, SOD activity and T-AOC content was similar in the five batches, and all three indices were significantly lower in W3 and W5 than in the other batches (P<0.01). Expression patterns of MDA content were opposite to those of CAT activity, SOD activity and T-AOC content. Protein profiles of all five batches were similar; the sizes of the predominant bands ranged from 20-110 kDa. We identified twenty-eight proteins with high scores in the database. These included heat shock proteins (HSPs), glucose-regulated protein 94, and arginine kinase

High-resolution spatial and genomic characterization of coral-associated microbial aggregates in the coral Stylophora pistillata

Bacteria commonly form aggregates in a range of coral species [termed coral-associated microbial aggregates (CAMAs)], although these structures remain poorly characterized despite extensive efforts studying the coral microbiome. Here, we comprehensively characterize CAMAs associated with Stylophora pistillata and quantify their cell abundance. Our analysis reveals that multiple Endozoicomonas phylotypes coexist inside a single CAMA. Nanoscale secondary ion mass spectrometry imaging revealed that the Endozoicomonas cells were enriched with phosphorus, with the elemental compositions of CAMAs different from coral tissues and endosymbiotic Symbiodiniaceae, highlighting a role in sequestering and cycling phosphate between coral holobiont partners. Consensus metagenome--assembled genomes of the two dominant Endozoicomonas phylotypes confirmed their metabolic potential for polyphosphate accumulation along with genomic signatures including type VI secretion systems allowing host association. Our findings provide unprecedented insights into Endozoicomonas-dominated CAMAs and the first direct physiological and genomic linked evidence of their biological role in the coral holobiont

How to Survive in the Shadow of Sharing Economy Giants: Business Model Innovation for Small and Medium-Sized Platforms

This study aims to explore how small and medium-sized platforms in the sharing economy can gain a competitive advantage in a market monopolized by giants. Taking China’s ride-hailing industry as an example, the k-means clustering method was employed to compare and analyze the characteristics and differences of 207 small and medium-sized platforms based on the framework of the business model canvas. An empirical typology comprising four representative successful business models of small and medium-sized ride-hailing platforms is extracted from the dimensions of key partners, value propositions, customer segmentation, and cost-revenue structure: high-end business platforms, Minibus-hailing platforms, Intercity carpool platforms, and Aggregation mode platforms. The results show that small and medium-sized platforms in the sharing economy can rely on their own characteristics and advantages to seek new potential development directions and gain development space by constructing value propositions and operating models that are different from industry giants. Specifically, it is necessary to further implement refined market identification and segmentation to continuously develop differentiated competitive advantages. In particular, more effective and open business strategies should be explored and practiced to greatly expand the network effects and business boundaries of small and medium-sized platforms, thereby significantly enhancing their competitiveness in the ride-hailing market. This study provides a new perspective on the reconstruction of market exchange in the sharing economy through the prism of small and medium-sized digital platforms and has certain important theoretical significance for supplementing and advancing existing research

Socioeconomic and Demographic Factors for Spousal Resemblance in Obesity Status in China

Introduction: The purposes of this study were to explore the resemblance in the weight status within couples with different family contextual factors and analyze the influence of the level of overweight or obesity of a spouse on that of the other spouse. Methods: The data were from the sixth National Health Service Survey of Henan Province in 2018. After screening, 7432 eligible couples were finally included. Socioeconomic and demographic factors were compared by the &chi;2 test or nonparametric test. The difference in the body mass index (BMI) of spouses was assessed by a t-test. The Pearson correlation coefficient and kappa value were used as indicators of consistency in weight status. A logistic regression analysis was used to further explore the effect of a spouse&rsquo;s level of overweight/obesity on that of the other spouse. Results: The results show that the prevalence of overweight/obesity in couples aged 20 or older is 33.76%. The Pearson correlation coefficient of the BMI within couples was 0.102 (95% CI: 0.076&ndash;0.120). The kappa coefficients suggested a low resemblance in the weight status within couples (k = 0.049, 95% CI: 0.031&ndash;0.069). Besides, the influence of the overweight/obesity status of the wives on that of the husbands (odds ratio (OR) = 1.411, 95% CI: 1.309&ndash;1.521) was slightly higher than that of the husbands on that of the wives (OR = 1.404, 95% CI: 1.302&ndash;1.514). Conclusions: We found that there was a moderate but significant resemblance in the body weight status between spouses, especially elderly couples with a low education level in rural areas. Health education activities for couple interventions can have a good effect of intervention

Cross-Talk between Transcriptome Analysis and Dynamic Changes of Carbohydrates Identifies Stage-Specific Genes during the Flower Bud Differentiation Process of Chinese Cherry (<i>Prunus pseudocerasus</i> L.)

Flower bud differentiation is crucial to reproductive success in plants. In the present study, RNA-Seq and nutrients quantification were used to identify the stage-specific genes for flower bud differentiation with buds which characterize the marked change during flower bud formation from a widely grown Chinese cherry (Prunus pseudocerasus L.) cultivar ‘Manaohong’. A KEGG enrichment analysis revealed that the sugar metabolism pathways dynamically changed. The gradually decreasing trend in the contents of total sugar, soluble sugar and protein implies that the differentiation was an energy-consuming process. Changes in the contents of D-glucose and sorbitol were conformed with the gene expression trends of bglX and SORD, respectively, which at least partially reflects a key role of the two substances in the transition from physiological to morphological differentiation. Further, the WRKY and SBP families were also significantly differentially expressed during the vegetative-to-reproductive transition. In addition, floral meristem identity genes, e.g., AP1, AP3, PI, AGL6, SEP1, LFY, and UFO demonstrate involvement in the specification of the petal and stamen primordia, and FPF1 might promote the onset of morphological differentiation. Conclusively, the available evidence justifies the involvement of sugar metabolism in the flower bud differentiation of Chinese cherry, and the uncovered candidate genes are beneficial to further elucidate flower bud differentiation in cherries

Failure Analysis Of Gate-All-Around Nanowire Field Effect Transistor Under Tlp Test

Electrostatic discharge (ESD) characters of Nanowire Field Effect Transistors have been tested and analyzed in detail in this paper. TLP (transmission line pulsing technique) and semiconductor characterization system have been used for experiments. The failure currents and leakage currents of Nanowire Field Effect Transistor are characterized. Also, physical insights and failure model are provided to analyze the failure mechanism

The construction and operational models of internet hospitals in China: a hospital-based survey study

Abstract Background China has empowered and continues to empower internet hospitals, which saw an increase in their development due to the pandemic, to fight against COVID-19. The construction and operational models of internet hospitals can be categorized as self-constructed and self-managed models, self-constructed and enterprise-run models, hospital and enterprise joint-owned models, and hosted by a third-party platform. Despite the growing importance of internet hospitals, there have been few systematic summaries of their construction and operational models. The primary purpose of the study was to understand the construction and operational models of internet hospitals in China. Methods Data was collected from 39 internet hospitals and 356 medical staff between September 2020 and April 2021, via internet hospital and hospital staff surveys. T-tests were used to compare the continuous variables, while Chi-square tests were employed to compare the proportions of categorical variables. The self-perception of the internet hospitals’ services was assessed using a 5-point Likert scale on 16 aspects and a root cause analysis was conducted to identify the root causes and influencing factors of current deficiencies experienced by internet hospitals. Results Among the 39 internet hospitals, 22 (56.4%) were self-constructed and self-managed. Compared to other models of Internet hospitals, self-constructed and self-managed hospitals had lower percentages of professionals providing online services (P = 0.006), numbers of doctors outside of the entity (P = 0.006), numbers of online nurses (P = 0.004), and the ratio of online nurses to offline doctors (P < 0.001). Of the 16 aspects evaluated with regards to the medical staff’s self-perception of the internet hospital services, the highest scores were given for fee transparency, fee rationality, travel cost capital, patience and responsibility, and consultation behaviors. The root causes included five aspects: human, channels, prices, services, and time. Conclusions While the self-constructed and self-managed model was found to be the most prevalent form of internet hospital in China, the different models of internet hospitals can have an impact on both the quantity and quality of online healthcare services. This study contributes to the existing literature on internet hospitals' construction and operational models, offering additional policy implications for telemedicine management

Autophagy in retinal ganglion cells in a rhesus monkey chronic hypertensive glaucoma model.

Primary open angle glaucoma (POAG) is a neurodegenerative disease characterized by physiological intraocular hypertension that causes damage to the retinal ganglion cells (RGCs). In the past, RGC damage in POAG was suggested to have been attributed to RGC apoptosis. However, in the present study, we applied a model closer to human POAG through the use of a chronic hypertensive glaucoma model in rhesus monkeys to investigate whether another mode of progressive cell death, autophagy, was activated in the glaucomatous retinas. First, in the glaucomatous retinas, the levels of LC3B-II, LC3B-II/LC3B-I and Beclin 1 increased as demonstrated by Western blot analyses, whereas early or initial autophagic vacuoles (AVi) and late or degraded autophagic vacuoles (AVd) accumulated in the ganglion cell layer (GCL) and in the inner plexiform layer (IPL) as determined by transmission electron microscopy (TEM) analysis. Second, lysosome activity and autophagosome-lysosomal fusion increased in the RGCs of the glaucomatous retinas, as demonstrated by Western blotting against lysosome associated membrane protein-1 (LAMP1) and double labeling against LC3B and LAMP1. Third, apoptosis was activated in the glaucomatous eyes with increased levels of caspase-3 and cleaved caspase-3 and an increased number of TUNEL-positive RGCs. Our results suggested that autophagy was activated in RGCs in the chronic hypertensive glaucoma model of rhesus monkeys and that autophagy may have potential as a new target for intervention in glaucoma treatment

Study on the Influence of Different Carbon Sources on Nitrogen and Phosphorus Removal with Micro-pressure swirl Reactor

To explore the suitable external carbon sources for Micro-pressure swirl Reactor (MPR), the experiment was run in parallel through two MPRs, using starch and glucose as carbon sources respectively. Research indicated that under the experimental operating conditions, using starch as the carbon source could more effectively promote the biological denitrification of the MPR system. Due to the structural characteristics of MPR and the slow degradation of starch, the demand for carbon source for denitrification was ensured during the operation cycle, so that the system obtained a better denitrification effect. The test results provided reference for the selection of the external carbon source of the MPR process

Average Daily Gain in Lambs Weaned at 60 Days of Age Is Correlated with Rumen and Rectum Microbiota

Colonization of gastrointestinal microbiota in mammals during early life is vital to host health. The objective of this study was to investigate whether lambs with high and low ADG have a different rumen and rectum microbial community. Thus, we investigated potential relationships between rumen and rectum microbiota and average daily gain (ADG) in weaned lambs. Sixteen lambs with similar body weights (7.63 ± 1.18 kg) were selected at 30 days of age. At 60 days of age, lambs were weaned, and ADG was calculated from 60 to 90 days. Then, two groups were generated: higher ADG (HG, 134.17 ± 13.48 g/day) and lower ADG (LG, 47.50 ± 19.51 g/day). Microbiota was evaluated at 30, 60, and 90 days of age. The final live weight and ADG at 90 days of age was higher (p p < 0.05) in the HG group for the 30 days vs. 90 days comparison and 60 days vs. 90 days comparison. Linear discriminant analysis effect size (LEfSe) analysis revealed a total of 18 bacterial biomarkers that are ADG-specific in the rumen and 35 bacterial biomarkers in the rectum. Meanwhile, 15 fungal biomarkers were found in the rumen and 8 biomarkers were found in the rectum. Our findings indicated that ADG is related to the rumen and rectum microbiota in lambs